Application of CRISPR-Cas9 genome editing technology in various fields: A review

Arif NM. Ansori; Yulanda Antonius; Raden JK. Susilo; Suhaila Hayaza; Viol D. Kharisma; Arli A. Parikesit; Rahadian Zainul; Vikash  Jakhmola; Taru  Saklani; Maksim  Rebezov; Md. Emdad  Ullah; Nikolai  Maksimiuk; Marina  Derkho; Pavel  Burkov

doi:10.52225/narra.v3i2.184

Authors

Arif NM. Ansori Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India; European Virus Bioinformatics Center, Jena, Germany https://orcid.org/0000-0002-1279-3904
Yulanda Antonius Faculty of Biotechnology, Universitas Surabaya, Surabaya, Indonesia https://orcid.org/0000-0002-4950-8134
Raden JK. Susilo Nanotechology Engineering Study Program, Faculty of Advanced Technology and Multidiscipline, Universitas Airlangga, Surabaya, Indonesia
Suhaila Hayaza Nanotechology Engineering Study Program, Faculty of Advanced Technology and Multidiscipline, Universitas Airlangga, Surabaya, Indonesia https://orcid.org/0000-0002-3920-6706
Viol D. Kharisma Doctoral Program of Mathematics and Natural Sciences, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia; Generasi Biologi Indonesia Foundation, Gresik, Indonesia
Arli A. Parikesit Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences (i3L), Jakarta, Indonesia https://orcid.org/0000-0001-8716-3926
Rahadian Zainul Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia
Vikash Jakhmola Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India https://orcid.org/0000-0002-8108-006X
Taru Saklani Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India
Maksim Rebezov Department of Scientific Research, V. M. Gorbatov Federal Research Center for Food Systems, Moscow, Russian Federation; Faculty of Biotechnology and Food Engineering, Ural State Agrarian University, Yekaterinburg, Russian Federation https://orcid.org/0000-0003-0857-5143
Md. Emdad Ullah Department of Chemistry, Mississippi State University, Mississippi, United States
Nikolai Maksimiuk Institute of Medical Education, Yaroslav-the-Wise Novgorod State University, Velikiy Novgorod, Russian Federation
Marina Derkho Institute of Veterinary Medicine, South Ural State Agrarian University, Troitsk, Russian Federation
Pavel Burkov Institute of Veterinary Medicine, South Ural State Agrarian University, Troitsk, Russian Federation

DOI:

https://doi.org/10.52225/narra.v3i2.184

Keywords:

CRISPR-Cas9, genome editing, medicine, molecular biology, molecular genetics

Abstract

CRISPR-Cas9 has emerged as a revolutionary tool that enables precise and efficient modifications of the genetic material. This review provides a comprehensive overview of CRISPR-Cas9 technology and its applications in genome editing. We begin by describing the fundamental principles of CRISPR-Cas9 technology, explaining how the system utilizes a single guide RNA (sgRNA) to direct the Cas9 nuclease to specific DNA sequences in the genome, resulting in targeted double-stranded breaks. In this review, we provide in-depth explorations of CRISPR-Cas9 technology and its applications in agriculture, medicine, environmental sciences, fisheries, nanotechnology, bioinformatics, and biotechnology. We also highlight its potential, ongoing research, and the ethical considerations and controversies surrounding its use. This review might contribute to the understanding of CRISPR-Cas9 technology and its implications in various fields, paving the way for future developments and responsible applications of this transformative technology.